0 avis
Mature Purkinje Cells Require the Retinoic Acid-Related Orphan Receptor-alpha (ROR alpha) to Maintain Climbing Fiber Mono-Innervation and Other Adult Characteristics
Archive ouverte
Edité par CCSD ; Society for Neuroscience -
International audience. Neuronal maturation during development is a multistep process regulated by transcription factors. The transcription factor ROR alpha (retinoic acid-related orphan receptor alpha) is necessary for early Purkinje cell (PC) maturation but is also expressed throughout adulthood. To identify the role of ROR alpha in mature PCs, we used Cre-lox mouse genetic tools in vivo that delete it specifically from PCs between postnatal days 10 -21. Up to 14 d of age, differences between mutant and control PCs were not detectable: both were mono-innervated by climbing fibers (CFs) extending along their well-developed dendrites with spiny branchlets. By week 4, mutant mice were ataxic, some PCs had died, and remaining PC soma and dendrites were atrophic, with almost complete disappearance of spiny branchlets. The innervation pattern of surviving ROR alpha -deleted PCs was abnormal with several immature characteristics. Notably, multiple functional CF innervation was reestablished on these mature PCs, simultaneously with the relocation of CF contacts to the PC soma and their stem dendrite. This morphological modification of CF contacts could be induced even later, using lentivirus-mediated depletion of rora from adult PCs. These data show that the late postnatal expression of ROR alpha cell-autonomously regulates the maintenance of PC dendritic complexity, and the CF innervation status of the PC (dendritic vs somatic contacts, and mono-innervation vs multi-innervation). Thus, the differentiation state of adult neurons is under the control of transcription factors; and in their absence, adult neurons lose their mature characteristics and acquire some characteristics of an earlier developmental stage.
Consulter en ligne
Suggestions
Du même auteur
