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Construction of Pseudomonas aeruginosa two-hybrid libraries for high-throughput assays.

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Bentzmann, Sophie, De | Bordi, Christophe

Edité par CCSD ; Humana Press/Springer Imprint -

International audience. In Pseudomonas aeruginosa, identification of new partners of a protein of interest could give precious clues to decipher a biological process in which this protein is involved. However, genes encoding for partners of a protein of interest are unknown and frequently scattered throughout the genome. We describe herein the construction and the use of pan-genomic bacterial two-hybrid libraries to identify new partners of a protein of interest encoded by P. aeruginosa.

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