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A synaptic F-actin network controls otoferlin-dependent exocytosis in auditory inner hair cells

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Vincent, Philippe F. Y. | Bouleau, Yohan | Petit, Christine | Dulon, Didier

Edité par CCSD ; eLife Sciences Publication -

International audience. We show that a cage-shaped F-actin network is essential for maintaining a tight spatial organization of Cav1.3 Ca 2+ channels at the synaptic ribbons of auditory inner hair cells. This F-actin network is also found to provide mechanosensitivity to the Cav1.3 channels when varying intracellular hydrostatic pressure. Furthermore, this F-actin mesh network attached to the synaptic ribbons directly influences the efficiency of otoferlin-dependent exocytosis and its sensitivity to intracellular hydrostatic pressure, independently of its action on the Cav1.3 channels. We propose a new mechanistic model for vesicle exocytosis in auditory hair cells where the rate of vesicle recruitment to the ribbons is directly controlled by a synaptic F-actin network and changes in intracellular hydrostatic pressure.

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