Syracuse

Contribution of phosphatases to adaptation of rhizobial symbiosis with legumes to phosphorus deficiency

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Lazali, Mohamed | Amenc, Laurie | Benadis, Chahinez | Brahimi, Samira | Abadie, Josiane | Zaman-Allah, Mainassara | Bargaz, Adnane | Drevon, Jean-Jacques

Edité par CCSD -

To understand the mechanisms used by legumes to improve their phosphorus use efficiency (PUE) for symbiotic nitrogen fixation under P-deficiency, an in situ RT-PCR methodology was used to localize and quantify the transcripts of candidate phosphatases genes in nodules of two common bean RILs 115 and 147 in hydro-aeroponic culture under deficient versus sufficient P supply. Our findings have revealed that expression of phytase and fructose 1,6 bisphosphatase (FBPase) genes and activities of the corresponding enzymes were positively correlated with increases both of the rhizobial symbiosis efficiency in use of P for N2 fixation and nodule O2 permeability. Under P-deficiency, this positive correlation was more significant for the P-efficient RIL115 than for theP-inefficient RIL147. It is concluded that these phosphatases play a role in adaptation to P-deficiency and are likely involved in nodule respiration linked to symbiotic nitrogen fixation.

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