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Quantitative measurement of permeabilization of living cells by terahertz attenuated total reflection

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Grognot, Marianne | Gallot, Guilhem

Edité par CCSD ; American Institute of Physics -

International audience. Using Attenuated Total Reflection imaging technique in the terahertz domain, we demonstrate non-invasive, non-staining real time measurements of cytoplasm leakage during permeabilization of epithelial cells by saponin. The terahertz signal is mostly sensitive to the intracellular protein concentration in the cells, in a very good agreement with standard bicinchoninic acid protein measurements. It opens the way to in situ real time dynamics of protein content and permeabilization in live cells.

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