Sensitive trimodal MRI-SERRS-fluorescence detection of cancer cells with stable magneto-plasmonic nanoprobes

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Carrouée, Ambre | Allard-Vannier, Emilie | Même, Sandra | Szeremeta, Frédéric | Beloeil, Jean-Claude | Chourpa, Igor

Edité par CCSD ; American Chemical Society -

International audience. Novel magneto-plasmonic nanoprobes were designed for multimodal diagnosis of cancer bycombination of Magnetic Resonance Imaging (MRI), Surface-Enhanced Resonance RamanScattering (SERRS) and fluorescence emission in the very near infrared (VNIR). A controlledelectrostatic assembly of silver nanoparticles (Ag NPs), superparamagnetic iron oxidenanoparticles (SPIONs), VNIR dye Nile blue (NB) and biopolymer chitosan (Chi) was used toformulate the AgIONs-Chi nanoprobes. The formulation protocol did not involve organicsolvents, was rapid and efficient as confirmed by magnetic sorting. The SERRS response of thenanoprobes was very intense and constant for days. It decreased linearly upon 1000-fold dilutionand was still recognizable at 0.1 nM NB concentration. After 30 days storage, the SERRS losswas less than 30% and the hydrodynamic size of the AgIONs-Chi in PBS remained below200nm. The gradual decrease of the ratio SERRS/fluorescence allowed monitoring the release ofthe fluorescent molecule upon long-term nanoprobe dissociation. The AgIONs-Chi exhibitedtwo-fold higher MRI contrast than that of commercially available SPION suspensions. Finally,the nanoprobes were actively uptaken by HeLa cancer cells and ensured trimodal MRI-SERRSfluorescencedetection of 10μL cell inclusions in cm-sized agarose gels used here as phantommodels of microtumors. The above results show that the magneto-plasmonic AgIONs-Chi arepromising substrates for SERRS analysis in solution and for multimodal imaging of cancer cells.

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