SAMBA, a plant-specific anaphase-promoting complex/cyclosome regulator is involved in early development and A-type cyclin stabilization

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Eloy, Nubia B. | Gonzalez, Nathalie | van Leene, Jelle | Maleux, Katrien | Vanhaeren, Hannes | de Milde, Liesbeth | Dhondt, Stijn | Vercruysse, Leen | Witters, Erwin | Mercier, Raphaël | Cromer, Laurence | Beemster, Gerrit T. S. | Remaut, Han | van Montagu, Marc C. E. | de Jaeger, Geert | Ferreira, Paulo C. G. | Inze, Dirk

Edité par CCSD ; National Academy of Sciences -

The anaphase-promoting complex/cyclosome (APC/C) is a large multiprotein E3 ubiquitin ligase involved in ubiquitin-dependent proteolysis of key cell cycle regulatory proteins, including the destruction of mitotic cyclins at the metaphase-to-anaphase transition. Despite its importance, the role of the APC/C in plant cells and the regulation of its activity during cell division remain poorly understood. Here, we describe the identification of a plant-specific negative regulator of the APC/C complex, designated SAMBA. In Arabidopsis thaliana, SAMBA is expressed during embryogenesis and early plant development and plays a key role in organ size control. Samba mutants produced larger seeds, leaves, and roots, which resulted from enlarged root and shoot apical meristems, and, additionally, they had a reduced fertility attributable to a hampered male gametogenesis. Inactivation of SAMBA stabilized A2-type cyclins during early development. Our data suggest that SAMBA regulates cell proliferation during early development by targeting CYCLIN A2 for APC/C-mediated proteolysis.

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