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Three BUB1 and BUBR1/MAD3-related spindle assembly checkpoint proteins are required for accurate mitosis in Arabidopsis
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The above article, first published online on Wiley Online Library (wileyonlinelibrary.com), and in New Phytologist 205: 202–215, has been retracted by agreement between the authors, the journal Editor-in-Chief, Alistair Hetherington, and John Wiley & Sons Ltd. Since publication of the above article, it has been brought to our attention that errors occurred in the construction of Figs 1 and 2(a); some components were inappropriately edited and duplicated, including the duplication and editing of images that first appeared in Caillaud et al. (2009), which were used by the authors as a basic template. Consequently, the integrity of the yeast two-hybrid experiments reported in the article is undermined, and, with agreement of all parties, the decision has been made to retract this article. We apologize for any inconvenience the publication of this work may have caused our readers. See : https://doi.org/10.1111/nph.14225. The spindle assembly checkpoint (SAC) is a refined surveillance mechanism which ensures that chromosomes undergoing mitosis do not segregate until they are properly attached to the spindle microtubules (MT). The SAC has been extensively studied in metazoans and yeast, but little is known about its role in plants. We identified proteins interacting with a MT-associated protein MAP65-3, which plays a critical role in organising mitotic MT arrays, and carried out a functional analysis of previously and newly identified SAC components. We show that Arabidopsis SAC proteins BUB3.1, MAD2, BUBR1/MAD3s and BRK1 interact with each other and with MAP65-3. We found that two BUBR1/MAD3s interacted specifically at centromeres. When stably expressed in Arabidopsis, BRK1 localised to the kinetochores during all stages of the mitotic cell cycle. Early in mitosis, BUB3.1 and BUBR1/MAD3.1 localise to the mitotic spindle, where MAP65-3 organises spindle MTs. A double-knockout mad3.1 mad3.2 mutant presented spindle MT abnormalities, chromosome misalignments on the metaphase plate and the production of lagging chromosomes and micronuclei during mitosis. We conclude that BRK1 and BUBR1/MAD3-related proteins play a key role in ensuring faithful chromosome segregation during mitosis and that their interaction with MAP65-3 may be important for the regulation of MT-chromosome attachment.
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