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Self-organization of the vesicular stomatitis virus nucleocapsid into a bullet shape.

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Desfosses, Ambroise | Ribeiro, Euripedes A | Schoehn, Guy | Blondel, Danielle | Guilligay, Delphine | Jamin, Marc | Ruigrok, Rob W H | Gutsche, Irina

Edité par CCSD ; Nature Publishing Group -

International audience. The typical bullet shape of Rhabdoviruses is thought to rely on the matrix protein for stabilizing the nucleocapsid coil. Here we scrutinize the morphology of purified and recombinant nucleocapsids of vesicular stomatitis virus in vitro. We elucidate pH and ionic strength conditions for their folding into conical tips and further growth into whole bullets, and provide cryo-electron microscopy reconstructions of the bullet tip and the helical trunk. We address conformational variability of the reconstituted nucleocapsids and the issue of constraints imposed by the binding of matrix protein. Our findings bridge the gap between the isolated nucleoprotein-RNA string in its form of an undulating ribbon, and the tight bullet-shaped virion skeleton.

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