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A Simple Method for 3D Analysis of Immunolabeled Axonal Tracts in a Transparent Nervous System

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Belle, Morgane | Godefroy, David | Dominici, Chloé | Heitz-Marchaland, Céline | Zelina, Pavol | Hellal, Farida | Bradke, Frank | Chédotal, Alain

Edité par CCSD ; Elsevier Inc -

International audience. Graphical Abstract Highlights Immunostaining and 3DISCO clearing: a powerful method for studying brain connections 3D analysis of axon guidance defects in midline mutant mice Unexpected roles for Slits and Netrin-1 in fasciculus retroflexus development In Brief Clearing techniques have recently been developed to look at mouse brains, but they are complex and expensive. Belle et al. now describe a simple procedure that combines immunolabeling, solvent-based clearing, and light-sheet fluores-cence microscopy. This technique allows large-scale screening of axon guidance defects and other developmental disor-ders in mutant mice.

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