Evaluation of propidium monoazide (PMA) treatment directly on membrane filter for the enumeration of viable but non cultivable Legionella by qPCR.

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Slimani, Sami | Robyns, Audrey | Jarraud, Sophie | Molmeret, Maëlle | Dusserre, Eric | Mazure, Céline | Facon, Jean Pierre | Lina, Gérard | Etienne, Jerome | Ginevra, Christophe

Edité par CCSD ; Elsevier -

International audience. A PMA (propidium monoazide) pretreatment protocol, in which PMA is applied directly to membrane filters, was developed for the PCR-based quantification (PMA-qPCR) of viable Legionella pneumophila. Using this method, the amplification of DNA from membrane-damaged L. pneumophila was strongly inhibited for samples containing a small number of dead bacteria.

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