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The role of distribution and orientation of collagen fibers in tissue development: study by means of double imaging by two-photon excited fluorescence and second harmonic generation microscopy

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Boryskina, Olena P. | Le Grand, Yann | Odin, Christophe | Fleury, Vincent

Edité par CCSD -

International audience. The TPEF and SHG imaging was used to study the distribution and orientation of collagen fibers in immature skin appendages on ex vivo chicken embryo skin samples in connection to mechanical properties of developing tissues. Our study has demonstrated that the orientation of collagen fibers in the feather buds may promote growth of feathers by creating the gradient of stiffness and thus triggering the pressure sensitive growth factors.

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