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From methylome to integrative analysis of tissue specificity

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de Bernonville, Thomas Duge | Daviaud, Christian | Chaparro, Cristian | Tost, Jörg | Maury, Stéphane

Edité par CCSD ; Humana Press/Springer Imprint -

International audience. DNA methylation is the most studied epigenetic mark in both plants and animals. The gold standard for assaying genome-wide DNA methylation at single-base resolution is whole-genome bisulfite sequencing (WGBS). Here, we describe an improved procedure for WGBS and original bioinformatic workflows applied to unravel tissue-specific variations of the methylome in relation to gene expression and accumulation of secondary metabolites in the medicinal plant Catharanthus roseus.

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