Method for tick-borne encephalitis virus detection in raw milk products

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Hennechart-Collette, Catherine | Gonzalez, Gaëlle | Fourniol, Lisa | Fraisse, Audrey | Beck, Cécile | Moutailler, Sara | Bournez, Laure | Dheilly, Nolwenn, M. | Lacour, Sandrine | Lecollinet, Sylvie | Martin-Latil, Sandra | Perelle, Sylvie

Edité par CCSD ; Elsevier -

International audience. The transmission of tick-borne encephalitis virus (TBEV) through food is rare, but can occur through the consumption of raw milk products from animals infected by tick bites. In 2020, France faced a TBEV outbreak linked to the consumption of unpasteurized goat cheese. The aim of this study was to develop and characterize a molecular method for the detection of TBEV in raw milk products based on the recent international standard PR ISO/DIS 16140-4. The TBEV recovery rates varied with the inoculation level and settings. The LOD50 and LOD95 of TBEV were 6.40 × 103 genome copies per g or per mL and 2.84 × 104 genome copies per g or per mL, respectively. The percentages of RT-qPCR inhibitions were lower than 75% and the murine norovirus (MNV-1), used as process control, was detected in all samples with a recovery rate higher than 1%, as recommended in ISO 15216. We conclude that the described method is appropriate to detect TBEV in raw milk products for routine diagnosis, and to assess potential health risks.

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