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Construction of a Yeast Artificial Chromosome Contig Spanning the Pseudoautosomal Region and Isolation of 25 New Sequence-Tagged Sites

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Slim, Rima | Le Paslier, Denis | Compain, Sylvie | Levilliers, Jacqueline | Ougen, Pierre | Billault, Alain | Donohue, Susan | Klein, David | Mintz, Liat | Bernheim, Alain | Cohen, Daniel | Weissenbach, Jean | Petit, Christine

Edité par CCSD ; Elsevier -

International audience. Thirty-one yeast artificial chromosomes (YACs) from the human pseudoautosomal region were identified by a combination of sequence-tagged site (STS) screenings and colony hybridizations, using a subtelomeric interspersed repetitive element mapping predominantly to the pseudoautosomal region. Twenty-five new pseudoautosomal STSs were generated, of which 4 detected restriction fragment length polymorphisms. A total of 33 STSs were used to assemble the 31 YACs into a single contiguous set of overlapping DNA fragments spanning at least 2.3 megabases of the pseudoautosomal region. In addition, four pseudoautosomal genes including hydroxyindole O-methyltransferase have been positioned on this set of fragments.

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