Afficher la couverture 'A protocol for ultrastructural study of Candida albicans biofilm using transmission electron microscopy | Mourer, Thierry' en grand format
/5

0 avis

A protocol for ultrastructural study of Candida albicans biofilm using transmission electron microscopy

Archive ouverte

Mourer, Thierry | Sachse, Martin | Gazi, Anastasia | D'Enfert, Christophe | Bachellier-Bassi, Sophie

Edité par CCSD ; Elsevier -

International audience. This protocol describes how to analyze C. albicans biofilm using transmission electron microscopy. We present two approaches to observe the ultrastructure of fungal cells within unperturbed biofilms, as well as an immunogold labeling procedure. This approach maintains the architecture of the fungal biofilm close to its native state by growing C. albicans biofilm on a plastic surface. After the freeze substitution procedure, classical transmission electron microscopy or electron tomography will allow the ultrastructural analysis of the microbial community.

Suggestions

Du même auteur

Involvement of amyloid proteins in the formation of biofilms in the pathogenic yeast Candida albicans

Archive ouverte | Mourer, Thierry | CCSD

International audience. Candida species represent a major fungal threat for human health. Within the Candida genus, the yeast Candida albicans is the most frequently incriminated species during episodes of candidias...

β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wall

Archive ouverte | Bekirian, Clara | CCSD

International audience. The cell wall of human fungal pathogens plays critical roles as an architectural scaffold and as a target and modulator of the host immune response. Although the cell wall of the pathogenic y...

The Pga59 cell wall protein is an amyloid forming protein involved in adhesion and biofilm establishment in the pathogenic yeast Candida albicans

Archive ouverte | Mourer, Thierry | CCSD

International audience. The human commensal fungus Candida albicans can attach to epithelia or indwelling medical devices and form biofilms, that are highly tolerant to antifungal drugs and can evade the immune resp...

Chargement des enrichissements...