Afficher la couverture 'APOBEC3C S188I Polymorphism Enhances Context-Specific Editing of Hepatitis B Virus Genome | Khalfi, Pierre' en grand format
/5

0 avis

APOBEC3C S188I Polymorphism Enhances Context-Specific Editing of Hepatitis B Virus Genome

Archive ouverte

Khalfi, Pierre | Suspène, Rodolphe | Caval, Vincent | Thiers, Valérie | Beauclair, Guillaume | Marchio, Agnès | Bekondi, Claudine | Amougou Atsama, Marie | Camengo-Police, Serge-Magloire | Noah Noah, Dominique | Njouom, Richard | Blanc, Hervé | Vallet, Thomas | Vignuzzi, Marco | Pineau, Pascal | Vartanian, Jean-Pierre

Edité par CCSD ; Oxford University Press -

International audience. Single-nucleotide polymorphism in APOBEC3C (resulting in a serine to isoleucine in position 188) is present in approximately 10% of African populations and greatly enhances restriction against human immunodeficiency virus-1 and simian immunodeficiency virus by improving dimerization and DNA processivity of the enzyme. In this study, we demonstrated in culture and in infected patients that hepatitis B virus (HBV) could be edited by APOBEC3CS188I. Using next-generation sequencing, we demonstrated that APOBEC3CS188I led to enhanced editing activity in 5ʹTpCpA→5ʹTpTpA context. This constitutes a new hallmark of this enzyme, which could be used to determine its impact on HBV or nuclear DNA.

Consulter en ligne

Suggestions

Du même auteur

Droplet digital PCR detects high rate of TP53 R249S mutants in cell-free DNA of middle African patients with hepatocellular carcinoma

Archive ouverte | Marchio, Agnès | CCSD

International audience. Hepatocellular carcinoma (HCC) is still a major killing malignancy in sub-Saharan Africa. Lifelong intoxication with aflatoxin B1 is considered as one of the primary causes of this situation....

Herpes Simplex Virus Type 1 Infection Disturbs the Mitochondrial Network, Leading to Type I Interferon Production through the RNA Polymerase III/RIG-I Pathway

Archive ouverte | Berry, Noémie | CCSD

International audience. Viruses have evolved a plethora of mechanisms to impair host innate immune responses. Herpes simplex virus type 1 (HSV-1), a double-stranded linear DNA virus, impairs the mitochondrial networ...

Cobalt chloride-mimicked hepatocyte cell hypoxia induces TREX1 leading to Hepatitis B virus restriction

Archive ouverte | Suspène, Rodolphe | CCSD

International audience. BackgroundRestriction factors are host cell proteins that play a role in limiting virus replication. They form part of the intrinsic immune system and function as a first line of defense agai...

Chargement des enrichissements...