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Crystallization and Preliminary Crystallographic Analysis of a Tetrameric Isolectin from Vicia villosa, Specific for the Tn Antigen

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Eiselé, Jean-Luc | Tello, Diana | Osinaga, Eduardo | Roseto, Alberto | Alzari, Pedro, M.

Edité par CCSD ; Elsevier -

International audience. Isolectin B4 isolated from Vicia villosa seeds is specific for the Tn antigen, a carcinoma-associated molecular marker. Crystals of the isolectin grown in the presence of carbohydrate are tetragonal, space group P 41 (or P43), with a = 91·3 Å, c = 151·7 Å and one tetramer in the asymmetric unit. The crystals diffract X-rays to 2·8 Å resolution and are suitable for high-resolution structural analysis.

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