Afficher la couverture 'Risk factors for HTLV-1 infection in Central Africa: A rural population-based survey in Gabon | Djuicy, Delia, Doreen' en grand format
/5

0 avis

Risk factors for HTLV-1 infection in Central Africa: A rural population-based survey in Gabon

Archive ouverte

Djuicy, Delia, Doreen | Mouinga-Ondémé, Augustin | Cassar, Olivier | Ramassamy, Jill-Léa | Idam Mamimandjiami, Antony | Bikangui, Rodrigue | Fontanet, Arnaud | Gessain, Antoine

Edité par CCSD ; Public Library of Science -

International audience. Background: Human T-Lymphotropic Virus type 1 (HTLV-1) is a human oncoretrovirus that infects at least 5 to 10 million people worldwide and is associated with severe diseases. Africa appears as the largest HTLV-1 endemic area. However, the risk factors for the acquisition of HTLV-1 remain poorly understood in Central Africa.Methods: We conducted an epidemiological survey between 2013 and 2017, in rural areas of 6 provinces of Gabon, in a rainforest environment. Epidemiological data were obtained and blood samples were collected after informed consent. Plasma were screened for HTLV-1 antibodies by ELISA and the positive samples were then tested by Western blot (WB). Genomic DNA derived from buffy-coat was subjected to two semi-nested PCRs amplifying either HTLV-1 env gene or LTR region fragments.Results: We recruited 2,060 individuals over 15 years old, including 1,205 men and 855 women (mean age: 49 years). Of these, 299 were found to be ELISA HTLV-1/2 seropositive. According to WB criteria, 136 were HTLV-1 (6.6%), 25 HTLV-1/2 (1.2%) and 9 HTLV seroreactive (0.4%). PCR results showed that 146 individuals were positive for at least one PCR: 104 for the env gene and 131 for the LTR region. Based on both serological and molecular results, 179 individuals were considered infected with HTLV-1, leading to an overall prevalence of 8.7%. The distribution of HTLV-1 infection was heterogeneous across the country. Based on multivariable analyses, female gender, increasing age, ethnicity (Pygmy) and multiple hospitalizations (more than 5 times) were found to be independent risk factors for HTLV-1 infection. Furthermore, a non-human primate bite appeared to be marginally associated with a higher risk of HTLV-1 infection.Conclusion: Based on state-of-the-art serological and molecular methods, we have demonstrated that rural adult populations in Gabon are highly endemic for HTLV-1. Our results regarding risk factors should lead to public health actions aiming to reduce HTLV-1 transmission.

Suggestions

Du même auteur

Epidemiology and Genetic Variability of HHV-8/KSHV among Rural Populations and Kaposi’s Sarcoma Patients in Gabon, Central Africa. Review of the Geographical Distribution of HHV-8 K1 Genotypes in Africa

Archive ouverte | Mamimandjiami, Antony Idam | CCSD

International audience. Human herpesvirus 8 (HHV-8) is the etiological agent of all forms of Kaposi’s sarcoma (KS). K1 gene studies have identified five major molecular genotypes with geographical clustering. This s...

Human T-Lymphotropic virus type 1 and human immunodeficiency virus co-infection in rural Gabon

Archive ouverte | Mouinga-Ondémé, Augustin | CCSD

International audience. IntroductionHuman T-cell lymphotrophic virus type-1 (HTLV-1) and human immunodeficiency virus (HIV-1) co-infection occur in many populations. People living with HIV-1 and infected with HTLV-1...

High prevalence of human T‐cell leukemia virus type‐1b genotype among blood donors in Gabon, Central Africa

Archive ouverte | Ramassamy, Jill-Léa | CCSD

International audience. BACKGROUNDThe African continent is considered to be the largest endemic area of HTLV‐1 infection, with at least several million infected individuals. Systematic screening of blood donors can ...

Chargement des enrichissements...