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Saporin-conjugated tetramers identify efficacious anti-HIV CD8+ T-cell specificities

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Leitman, Ellen | Palmer, Christine | Buus, Søren | Chen, Fabian | Riddell, Lynn | Sims, Stuart | Klenerman, Paul | Saez-Cirion, Asier | Walker, Bruce | Hess, Paul | Altfeld, Marcus | Matthews, Philippa | Goulder, Philip

Edité par CCSD ; Public Library of Science -

International audience. Antigen-specific T-cells are highly variable, spanning potent antiviral efficacy and damaging auto-reactivity. In virus infections, identifying the most efficacious responses is critical to vaccine design. However, current methods depend on indirect measures or on ex vivo expanded CTL clones. We here describe a novel application of cytotoxic saporin-conjugated tetramers to kill antigen-specific T-cells without significant off-target effects. The relative efficacy of distinct antiviral CD8+ T-cell specificity can be directly assessed via antigen-specific CD8+ T-cell depletion. The utility of these reagents is demonstrated here in identifying the CD8+ T-cell specificity most effective in preventing HIV progression in HIV-infected HLA-B*27-positive immune controllers.

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