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Best practices and benchmarks for intact protein analysis for top-down mass spectrometry

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Donnelly, Daniel | Rawlins, Catherine | Dehart, Caroline | Fornelli, Luca | Schachner, Luis | Lin, Ziqing | Lippens, Jennifer | Aluri, Krishna | Sarin, Richa | Chen, Bifan | Lantz, Carter | Jung, Wonhyeuk | Johnson, Kendall | Koller, Antonius | Wolff, Jeremy | Campuzano, Iain | Auclair, Jared | Ivanov, Alexander | Whitelegge, Julian | Paša-Tolić, Ljiljana | Chamot-Rooke, Julia | Danis, Paul | Smith, Lloyd | Tsybin, Yury | Loo, Joseph | Ge, Ying | Kelleher, Neil | Agar, Jeffrey

Edité par CCSD ; Nature Publishing Group -

International audience. One gene can give rise to many functionally distinct proteoforms, each of which has a characteristic molecular mass. Top-down mass spectrometry enables the analysis of intact proteins and proteoforms. Here members of the Consortium for Top-Down Proteomics provide a decision tree that guides researchers to robust protocols for mass analysis of intact proteins (antibodies, membrane proteins and others) from mixtures of varying complexity. We also present cross-platform analytical benchmarks using a protein standard sample, to allow users to gauge their proficiency.

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