Guidelines for the use of flow cytometry and cell sorting in immunological studies
Archive ouverte
Cossarizza, Andrea | Chang, Hyun-Dong | Radbruch, Andreas | Andrä, Immanuel | Annunziato, Francesco | Bacher, Petra | Barnaba, Regina | Battistini, Luca | Bauer, Wolfgang | Baumgart, Sabine | Becher, Burkhard | Beisker, Wolfgang | Berek, Claudia | Blanco, Alfonso | Borsellino, Giovanna | Boulais, David | Brinkman, Ryan R | Büscher, Martin | Busch, Dirk | Bushnell, Timothy P | Cao, Xuetao | Cavani, Andrea | Chattopadhyay, Pratip K | Cheng, Qingyu | Chow, Sue | Clerici, Mario | Cooke, Anne | Cosma, Antonio | Cosmi, Lorenzo | Dang, van Duc | Davies, Derek | de Biasi, Mor | del Zotto, Genny | Della Bella, Silvia | Dellabona, Paolo | Deniz, Günnur | Dessing, Mark | Diefenbach, Andreas | Di Santo, James, P. | Dieli, Francesco | Dolf, Bo | Donnenberg, Vera S | Dörner, Thomas | Ehrhardt, Götz R A | Endl, Bo | Engel, Pablo | Engelhardt, Britta | Esser, Charlotte | Everts, Bart | Falk, Christine | Fehniger, Todd A | Filby, Andrew | Simon, Simon | Follo, Marie | Förster, Bo | Foster, John | Foulds, John | Frenette, Paul S | Galbraith, Ari | Garbi, Bo | García-Godoy, Maria Dolores | Ghoreschi, Kamran | Gibellini, Mor | Goettlinger, Christoph | Goodyear, Carl S | Gori, Andrea | Grogan, Francisco | Gross, Mor | Grützkau, Andreas | Grummitt, Daryl | Hahn, Alexander | Hammer, Quirin | Hauser, Anja E | Houston, David | Hedley, David | Herrera, Guadalupe | Herrmann, Alexander | Hiepe, Falk | Holland, Bo | Hombrink, Pleun | Houston, Jessica P | Hoyer, Bimba F | Huang, Ton | Hunter, Christopher A | Iannone, Anna | Jäck, Hans-Martin | Jávega, Beatriz | Jonjic, Stipan | Juelke, Kerstin | Jung, Steffen | Kaiser, Toralf | Kalina, Tomas | Keller, Baerbel | Khan, Srijit | Kienhöfer, Alexander | Kroneis, Thomas | Kunkel, Désirée | Kurts, Christian | Kvistborg, Pia | Lannigan, Virginia | Lantz, Olivier | Larbi, Anis, A | Leibundgut-Landmann, Salomé | Leipold, Michael D | Levings, Megan K | Litwin, Virginia | Liu, Yanling | Lohoff, Michael | Lombardi, Giovanna | Lopez, Lilly | Lovett-Racke, Amy | Lubberts, Erik | Ludewig, Burkhard | Lugli, Enrico | Maecker, Holden T | Martrus, Gloria | Matarese, Giuseppe | Maueröder, Alexander | Mcgrath, Mairi | Mcinnes, Iain | Mei, Henrik E | Melchers, Fritz | Melzer, Susanne | Mielenz, Dirk | Mills, Kingston | Mjösberg, Jenny | Moore, Jonni | Moran, Barry | Moretta, Alessandro | Moretta, Lorenzo | Mosmann, David | Müller, Susann | Müller, Werner | Münz, Christian | Multhoff, Gabriele | Munoz, Alexander | Murphy, Kenneth M | Nakayama, Toshinori | Nasi, Milena | Neudörfl, Christine | Nolan, John | Nourshargh, Sussan | O'Connor, José-Enrique | Ouyang, Francisco | Oxenius, Annette | Palankar, Raghav | Panse, Isabel | Peterson, Peter | Peth, Christian | Petriz, Jordi | Philips, Daisy | Pickl, Winfried | Piconese, Regina | Pinti, Marcello | Pockley, a Graham | Podolska, Alexander | Pucillo, Carlo | Quataert, David | Radstake, Timothy R D J | Rajwa, Bartek | Rebhahn, David | Recktenwald, Diether | Remmerswaal, Ester B M | Rezvani, Katy | Rico, Laura G | Robinson, J Paul | Romagnani, Chiara | Rubartelli, Anna | Ruland, Jürgen | Sakaguchi, Shimon | Sala-De-Oyanguren, Francisco | Samstag, Yvonne | Sanderson, Sharon | Sawitzki, Birgit | Scheffold, Alexander | Schiemann, Matthias | Schildberg, Frank | Schimisky, Esther | Schmid, Stephan A | Schmitt, Steffen | Schober, Kilian | Schüler, Thomas | Schulz, Axel Ronald | Schumacher, Ton | Scotta, Cristiano | Shankey, T Vincent | Shemer, Anat | Simon, Anna-Katharina | Spidlen, Josef | Stall, Alan M | Stark, Regina | Stehle, Christina | Stein, Merle | Steinmetz, Tobit | Stockinger, Hannes | Takahama, Yousuke | Tarnok, Attila | Tian, Zhigang | Toldi, Gergely | Tornack, Julia | Traggiai, Elisabetta | Trotter, Joe | Ulrich, Paulo | van Der Braber, Marlous | van Lier, René a W | Veldhoen, Marcello | Vento-Asturias, Salvador | Vieira, Paulo | Voehringer, David | Volk, Hans-Dieter | von Volkmann, Konrad | Waisman, Ari | Walker, Rachael | Ward, Michael D | Warnatz, Klaus | Warth, Sarah | Watson, James | Watzl, Carsten | Wegener, Leonie | Wiedemann, Annika | Wienands, Jürgen | Willimsky, Gerald | Wing, James | Wurst, Peter | Yu, Liping | Yue, Alice | Zhang, Qianjun | Zhao, Yi | Ziegler, Susanne | Zimmermann, Jakob
Edité par
CCSD ; Wiley-VCH Verlag -
International audience.
The classical model of hematopoiesis established in the mouse postulates that lymphoid cells originate from a founder population of common lymphoid progenitors. Here, using a modeling approach in humanized mice, we showed that human lymphoid development stemmed from distinct populations of CD127(-) and CD127(+) early lymphoid progenitors (ELPs). Combining molecular analyses with in vitro and in vivo functional assays, we demonstrated that CD127(-) and CD127(+) ELPs emerged independently from lympho-mono-dendritic progenitors, responded differently to Notch1 signals, underwent divergent modes of lineage restriction, and displayed both common and specific differentiation potentials. Whereas CD127(-) ELPs comprised precursors of T cells, marginal zone B cells, and natural killer (NK) and innate lymphoid cells (ILCs), CD127(+) ELPs supported production of all NK cell, ILC, and B cell populations but lacked T potential. On the basis of these results, we propose a "two-family" model of human lymphoid development that differs from the prevailing model of hematopoiesis.
