Hepatitis C virus infection protein network.

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de Chassey, B. | Navratil, V. | Tafforeau, L. | Hiet, M. S. | Aublin-Gex, A. | Agaugué, S. | Meiffren, G. | Pradezynski, F. | Faria, B. F. | Chantier, T. | Le Breton, M. | Pellet, J. | Davoust, N. | Mangeot, P. E. | Chaboud, A. | Penin, F. | Jacob, Y. | Vidalain, P. O. | Vidal, M. | André, P. | Rabourdin-Combe, C. | Lotteau, V.

Edité par CCSD ; EMBO Press -

International audience. A proteome-wide mapping of interactions between hepatitis C virus (HCV) and human proteins was performed to provide a comprehensive view of the cellular infection. A total of 314 protein-protein interactions between HCV and human proteins was identified by yeast two-hybrid and 170 by literature mining. Integration of this data set into a reconstructed human interactome showed that cellular proteins interacting with HCV are enriched in highly central and interconnected proteins. A global analysis on the basis of functional annotation highlighted the enrichment of cellular pathways targeted by HCV. A network of proteins associated with frequent clinical disorders of chronically infected patients was constructed by connecting the insulin, Jak/STAT and TGFbeta pathways with cellular proteins targeted by HCV. CORE protein appeared as a major perturbator of this network. Focal adhesion was identified as a new function affected by HCV, mainly by NS3 and NS5A proteins.

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