Real-time PCR detection of gyrA and parC mutations in Streptococcus pneumoniae.

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Page, S. | Vernel-Pauillac, F. | O'Connor, O. | Bremont, S. | Charavay, F. | Courvalin, P. | Goarant, C. | Le Hello, S.

Edité par CCSD ; American Society for Microbiology -

International audience. Fluoroquinolone resistance in Streptococcus pneumoniae mainly involves stepwise mutations predominantly in the parC and gyrA genes. We have developed a single-run real-time PCR assay for detection of the four most common mutations in the quinolone resistance-determining regions of these genes. This assay provides a useful tool for both clinical and epidemiological use.

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