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Cryo-EM Visualization of Neuronal Particles Inside Microtubules

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Gory-Fauré, Sylvie | Delaroche, Julie | Cuveillier, Camille | Delphin, Christian | Arnal, Isabelle

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International audience. Neuronal microtubules have long been known to contain intraluminal particles, called MIPs (microtubule inner proteins), most likely involved in the extreme stability of microtubules in neurons. This chapter describes a cryo-electron microscopy-based assay to visualize microtubules containing neuronal MIPs. We present two protocols to prepare MIPs-containing microtubules, using either in vitro microtubule polymerization assays or extraction of microtubules from mouse hippocampal neurons in culture.

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