0 avis
OR13-003 - TNFRSF11A molecular defects cause autoinflammatory disorders
Archive ouverte
Edité par CCSD -
International audience. IntroductionHereditary recurrent fevers (HRF) are autoinflammatory disorders whose etiology remains unknown in many cases.ObjectivesTo identify a new HRF geneMethodsComparative genomic hybridization (CGH, 385K array) was performed in the proband. TNFRSF11A was screenedby Sanger sequencing in other patients. TNFRS11A expression was quantified by fluorescence-activated cellsorter analysis (FACS). NF-k B activation was assessed using a luciferase assay in HEK293 cells transfected withplasmids encoding wild-type and mutated TNFRSF11A.ResultsArray-CGH analysis performed in a patient with multiple congenital anomalies and a recurrent fever syndromerevealed a de novo heterozygous chromosomal rearrangement encompassing a duplication of TNFRSF11A. Thistransmembrane receptor binds the TNFSF11 cytokine, activates NF-k B signaling, and regulates fever in rodents,consistent with a possible role in HRF. TNFRSF11A screening in other patients with genetically-unexplainedHRF revealed a heterozygous frameshift mutation in a patient and her affected mother. The mutated protein isexpressed at similar levels as the normal receptor on leukocytes. Most importantly, this mutation results in a gainof function on NF-k B signaling, since the mutated protein is more responsive to TNFSF11 stimulation than the wild-type receptor. Since TNFRSF11A (also known as RANK) was previously known for its key role in osteoclastogenesis, the medical history of our patients was reassessed and revealed minor symptoms also found in patients with TNFRSF11A-associated bone disorders.ConclusionThe implication of TNFRSF11A in HRF reveals a key role of this receptor in autoinflammation and opens up newfields of research at the crossroads between bone metabolism and innate immunity.
Consulter en ligne
Suggestions
Du même auteur
