Contribution of annexin 2 to the architecture of mature endothelial adherens junctions.. Contribution of annexin 2 to the architecture of mature endothelial adherens junctions.: Annexin 2 stabilizes interendothelial junctions

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Heyraud, Stéphanie | Jaquinod, Michel | Durmort, Claire | Dambroise, Emilie | Concord, Evelyne | Schaal, Jean Patrick | Huber, Philippe | Gulino-Debrac, Danielle

Edité par CCSD ; American Society for Microbiology -

International audience. The vascular endothelial cadherin (VE-cad)-based complex is involved in the maintenance of vascular endothelium integrity. Using immunoprecipitation experiments, we have demonstrated that, in confluent human umbilical vein endothelial cells, the VE-cad-based complex interacts with annexin 2 and that annexin 2 translocates from the cytoplasm to the cell-cell contact sites as cell confluence is established. Annexin 2, located in cholesterol rafts, binds to both the actin cytoskeleton and the VE-cad-based complex so the complex is docked to cholesterol rafts. These multiple connections prevent the lateral diffusion of the VE-cad-based complex, thus strengthening adherens junctions in the ultimate steps of maturation. Moreover, we observed that the down-regulation of annexin 2 by small interfering RNA induces a delocalization of VE-cad from adherens junctions and consequently a destabilization of these junctions. Furthermore, our data indicate that the decoupling of the annexin 2/p11 complex from the VE-cad-based junction, triggered by vascular endothelial growth factor treatment, facilitates the switch from a quiescent to an immature state.

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