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Oocyte expression with injection of purified T7 RNA polymerase.

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Altafaj, Xavier | Joux, Nathalie | Ronjat, Michel | de Waard, Michel

Edité par CCSD ; Humana Press/Springer Imprint -

International audience. The Xenopus oocyte is a widely used system for protein expression. Investigators have had the choice between two different techniques: injection into the cytoplasm of in vitro transcribed complementary RNA (cRNA) or injection into the nucleus of complementary DNA (cDNA). We report on a third expression technique that is based on the combined injection of cDNA and purified T7 RNA polymerase directly into the cytoplasm of oocytes.

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