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A set of baculovirus transfer vectors for screening of affinity tags and parallel expression strategies.

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Abdulrahman, Wassim | Uhring, Muriel | Kolb-Cheynel, Isabelle | Garnier, Jean-Marie | Moras, Dino | Rochel, Natacha | Busso, Didier | Poterszman, Arnaud

Edité par CCSD ; Elsevier Masson -

International audience. We report a set of baculovirus transfer vectors for parallel expression of proteins in fusion with a panel of affinity tags including GST, protein A, thioredoxin, CBP, and FLAG. This suite includes vectors to generate recombinant baculovirus by homologous recombination in insect cells or using the Bac-to-Bac technology. An application of the vector suite approach to the vitamin D receptor (VDR), a protein mainly expressed as inclusion bodies in Escherichia coli, is presented. We found that expression in fusion with GST and protein A provided an efficient compromise of excellent purification with acceptable yields and costs.

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