Automated recombinant protein expression screening in Escherichia coli.

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Automated recombinant protein expression screening in Escherichia coli.

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Busso, Didier | Stierlé, Matthieu | Thierry, Jean-Claude | Moras, Dino

Edité par CCSD ; Humana Press/Springer Imprint -

International audience. To fit the requirements of structural genomics programs, new as well as classical methods have been adapted to automation. This chapter describes the automated procedure developed within the Structural Biology and Genomics Platform, Strasbourg for performing recombinant protein expression screening in Escherichia coli. The procedure consists of parallel competent cells transformation, cell plating, and liquid culture inoculation, implemented for up to 96 samples at a time.

Langue: en

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