Isolation and characterization of microsatellites in a perennial Apiaceae, Eryngium alpinum L

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Gaudeul, M. | Naciri-Graven, Y. | Gauthier, P. | Pompanon, F.

Edité par CCSD ; Wiley-Blackwell -

International audience. In order to investigate the genetic structure in an endangered Alpine plant (Eryngium alpinum L.), we developed microsatellites. Two different approaches were used: an enrichment protocol and the classical technique of hybridization on nylon membranes. We identified 25 loci, 13 of which revealed to be polymorphic. The polymorphism was rather low (2-6 alleles; H-E = 0.49 +/- 0.16), probably due to the short size of microsatellites (6-10 dinucleotide repeats) and to the fine spatial scale investigated. However, these markers are expected to provide a new insight about the genetic processes at work within and among E. alpinum populations.

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