Protocol for isolating CD163+ Kupffer cells from human liver resections

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Roca Suarez, Armando, Andres | Plissonnier, Marie‐laure | Michelet, Maud | Dubois, Anaëlle | Heintz, Sarah | Saez-Palma, Maria | Delphin, Marion | Bordes, Isabelle | Molle, Jennifer | Diederichs, Audrey | Rodà, Mélanie | Combe, Emmanuel | Mougené, Léa | Giraud, Guillaume | Grand, Xavier | Rivoire, Michel | Levrero, Massimo | Testoni, Barbara | Zoulim, Fabien

Edité par CCSD ; Elsevier -

International audience. The liver microenvironment contains a wide variety of monocyte and macrophage populations. Here, we present a protocol for the specific isolation of liver-resident macrophages, known as Kupffer cells (KCs), from human liver resections. We describe steps for dissociating human liver tissues, separating non-parenchymal cells into fractions by a 2-phase iodixanol gradient, and positive selection of KCs based on the expression of CD163. We then provide instructions for validating the procedure by immunofluorescence to detect CD163.

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