Updated distribution of kdr mutations associated with resistance to pyrethroids in European populations of Aedes albopictus

Archive ouverte

Pichler, Verena | Valadas, Vera | Horvath, Cintia | Akiner, Muhammed | Capinha, César | Schaffner, Francis | Vasquez, Marlen | Bouyer, Jérémy | Caputo, Beniamino | Della Torre, Alessandra | Pinto, João

Edité par CCSD -

International audience. Introduction and Objectives : Resistance to pyrethroids (PR) - the only insecticides authorized in case of autochthonous arboviruses transmission in Europe - has been reported in Aedes albopictus populations from Italy and Spain. Mutations at positions 1016, 1532 and 1534 of the voltage-gated sodium-channel (VGSC) gene are the most strongly associated with target-site PR in the species. Results of a European-wide investigation revealed a widespread presence of allele 1016G in Ae. albopictus, with frequencies up to 45% in Italy. Although four alleles at positions 1532 (T) and 1534 (C/L/S) have been described worldwide, in Europe Ae. albopictus, monitoring of 1534C was carried out only for Ae. albopictus from Italy and Greece, and the allele was found at frequencies up to 68% in the latter. Here, we show the results of a Europeanwide effort to map 1532 and 1534 mutations in Ae. albopictus.Materials and Methods : Genotyping was performed by Allelic-Specific PCR (AS-PCR) in 19 European Ae. albopictus populations. Results were validated by sequencing in a sub-sample of specimens.Results, Discussion and Conclusion : Among the 1,731 specimens genotyped, allele 1534C was found in Spain, Georgia, Romania, and Albania at frequencies <5%, in Greece at frequencies up to 47%, as well as in the recently colonized Cyprus (84%), suggesting the origin of the invasive population from Greece. Allele 1534L was only found in Rome (3.1%). Allele 1532T was detected in Spain, France, Italy, Greece, Albania and Turkey at 4%-25% frequencies. The high frequencies of allele 1016G in Italy and 1534C in Greece and the intense connections between the two countries open the possibility of cross-over between haplotypes carrying the two alleles resulting potentially in a strongly enhanced resistance phenotype, a risk which stresses further the need to carefully monitor resistance genotypes.

• Description
• Sujet/Public
• Outil
• Outil d'anticipation
• Mémoire et thèse
• Gestion