Syracuse

Massive premature cleavage and polyadenylation in a PRP31 mutant at 37°C

Archive ouverte

Courtois, Charles | Mathieu, Olivier | Pélissier, Thierry | Bourguet, Pierre

Edité par CCSD -

International audience. In Arabidopsis thaliana, heat stress leads to a release of transcriptional repression of heterochromatin, independently of changes in patterns of common epigenetic marks. In a search for factors controlling this process, we have identified the zen3 mutation affecting the PRP31 gene, which encodes an essential splicing factor. In response to heat stress, zen3 plants show compromised reactivation of heterochromatin transcription and mis-expression of thousands of genes. Transcriptome analysis reveals that this is associated with widespread early polyadenylation of mRNAs (PCPA for Premature Cleavage and PolyAdenylation) in zen3, suggesting an important role for PRP31 in protecting mRNAs from PCPA. Indeed, our survival tests attest of an essential function of PRP31. In order to assess potential role of PRP31 in protecting against PCPA, we used an artificial microRNAs implementation approach to discern a specific function in response to heat stress from a thermo-sensitive mutation. The detection of a GFP tag fused to wild type PRP31 or the mutant form by microscopy showed a clear altered nuclear distribution in zen3 mutant.

• Description
• Sujet/Public
• Outil
• Outil d'anticipation
• Mémoire et thèse
• Gestion