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Survivability of LACTIFERM® as a direct fed microbials for calves: application to feeding practices
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Edité par CCSD ; Wageningen Academic Publishers -
Session 49. Nutrition in health and welfare of ruminants Poster 16. International audience. Under the last couple of decades, direct fed microbials (DFM) have been increasingly used in animal production.In young ruminants, DFM were linked to improvement of body weight and size, feed efficiency, resistance todiseases, establishment of the rumen microbiota as well as development of rumen papillae and function. However,prior to reach the gastrointestinal tract and exert their beneficial effects, DFM need to survive to manutention,transport, and wide diversity of feeding practices. In this study, we investigate the survivability of LACTIFERM®(Novonesis, Denmark), a commercial product for calves, in milk replacer at room temperature, in milk replaceracidified with formic acid as well as its compatibility with halofuginone, a veterinary drug used for cryptosporidiosisin newborn calves. The capacity of Enterococcus faecium 669, the active strain from the LACTIFERM®product, to grow in a complex medium mimicking the composition of rumen environment after a meal or in purerumen fluid was assessed by optical density. LACTIFERM® displayed an excellent survivability and growth abilityin milk replacer left at room temperature over 24 hours as well as excellent stability on acidified milk replacerwith formic acid. Simulation of halofuginone supplementation after a meal of milk replacer did not impact themultiplication of LACTIFERM® at calf stomach temperature. At last, E. faecium 669 demonstrated the abilityto grow in rumen containing media. Altogether, this study suggests that LACTIFERM® possess the capacity todeploy its full beneficial properties in calf rumen even while handled with various feeding practices.