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The volatolomic signature differs between primiparous and multiparous cows across different matrices
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Edité par CCSD ; Wageningen Academic Publishers -
Session 13. Sensing Physiology: Tools towards optimising livestock husbandryPrésentation orale. International audience. Non-invasive phenotyping of cattle is becoming of increasing importance to develop new tools to study and individuallymanage farm animals. We explored the effect of the dairy cows’ parity on the volatolomics of severalnon-invasive matrices such as breath, ruminal liquid, urine, faeces and sweat. Ten primiparous (PRIM, 73 ± 11days in milk, 22.3 ± 3.31 kg/d milk yield, 618 ± 41 kg BW, 1.5 ± 0.20 BCS) and 6 multiparous (MULT, 2nd lactation,23.0 ± 1.59 kg/d milk yield, 76 ± 11 days in milk, 642 ± 49 kg BW, 1.4 ± 0.20 BCS) Holstein cows werefed ad libitum a high-fibre diet (53% FDN, 12% CP) during 5 weeks. Cows were sampled at the end of weeks 4and 5, and the samples were stored until analysis (-80°C) except for breath, which was stored at room temperatureand analysed within 24h after sampling. Samples were thawed (37°C, 10 min), heated (45°C, 30 min) and theirheadspace analysed through selected-ion flow-tube mass spectrometry (SIFT-MS) in full mode scan (positiveand negative ions). The parity effect on the volatolome was explored through Partial-least square discriminantanalysis considering weeks 4 and 5 independently. The breath volatolome discriminated the parity only in week4 (R2Y=0.96, Q2=0.83) being the best discrimination model. The faeces did not yield any valid model. The restof the matrices always yielded a valid model, with acceptable predictive ability observed for ruminal liquid onweek 4 (Q2=0.59), and urine (Q2 = 0.58) and sweat (Q2 = 0.59) on week 5. Our results suggest that metabolic anddigestive differences between primiparous and multiparous lactating cows were caught by the volatolome of thesematrices, and so future studies should consider the effect of the parity.
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