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Screening of Mediterranean agro-industrial by-products on in vitro ruminal fermentation
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Edité par CCSD ; Wageningen Academic Publishers -
Session 5. Innovative and emerging feed and forage resources. International audience. The utilization of polyphenol-rich agro-industrial by-products for ruminant diet would be a sustainable and circularoption to mitigate pollutant emissions. In this study, 16 by-products sourced from Greece, Italy, and Francewere evaluated at 10% inclusion level in a basal diet using an in vitro ruminal fermentation assay. Dry matterdegradability (DMD), gas production (CH4, CO2), volatile fatty acids (VFAs), and ammonia (NH3) concentrationswere monitored. A mixed-model ANOVA test was utilized to assess the effects of each by-product relative toone another. Additionally, a Dunnett post hoc test was conducted to compare the by-products against the control,comprised solely of the basal diet. Results revealed significant compared to control diet reductions in CH4 productionwith French grape, Italian hazelnut, and Greek pomegranate by-products (p<0.001). French grape, Italianand Greek pomegranate led to an increased CO2/CH4 ratio (p<0.01) and decreased NH3 production (p<0.05)compared to control diet. In addition, the French grape by-product significantly reduced DMD (p<0.05), withoutaffecting VFAs production. Furthermore, this by-product demonstrates a decrease in CH4 and NH3 production(p<0.001) compared to the control. These findings suggest a shift in fermentative pathways from CH4 to CO2,affecting methanogenic microorganisms. Despite the lower DMD observed, the French grape by-product emergesas the best candidate for mitigating pollutant emissions.