0 avis
Identification of Resistance QTLs to Black Leaf Streak Disease (Due to Pseudocercospora fijiensis) in Diploid Bananas (Musa acuminata)
Archive ouverte
The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/horticulturae10060608/s1, Figure S1: Location of the recombinations detected on each parent (KNO and IDN110) on the physical map of DH Pahang reference genome version 4 [37]; Figure S2: Manhattan plots of KNO (a.) and IDN110 (b.) for the 16 phenotypic variables; Table S1: For all hybrids, BLUP values of the phenotypic variables and genotypes for the 2084 non-redundant markers; Table S2: List of the genes or putative genes identified in the four main QTLs of IDN110.. International audience. Black Leaf Streak Disease (BLSD), caused by the fungus Pseudocercospora fijiensis, is a recent pandemic and the most economically and environmentally important leaf disease of banana. To assist breeding of varieties with durable resistance to the rapidly evolving P. fijiensis, we used a diploid genitor 'IDN 110' with partial resistance to BLSD to search for QTLs. We assessed diploid progeny of 73 hybrids between 'IDN 110' and the diploid cultivar 'Khai Nai On', which is susceptible to BLSD. Hybrids were phenotyped with artificial inoculation under controlled conditions. This method allowed us to focus on resistance in the early stages of the interaction already identified as strongly influencing BLSD epidemiology. Progeny were genotyped by sequencing. As both parents are heterozygous for large reciprocal translocations, the distribution of recombination was assessed and revealed regions with low recombination rates. Fourteen non-overlapping QTLs of resistance to BLSD were identified of which four main QTLs from the 'IDN110' parent, located on chromosomes 06, 07, 08, and 09, were shown to be of interest for marker-assisted selection. Genes that underline those four QTLs are discussed in the light of previous literature.
Consulter en ligne
Suggestions
Du même auteur
