Regulation of stress-induced sleep perturbations by dorsal raphe VGLUT3 neurons in male mice

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Henderson, Fiona | Dumas, Sylvie | Gangarossa, Giuseppe | Bernard, Véronique | Pujol, Marine | Poirel, Odile | Pietrancosta, Nicolas | El Mestikawy, Salah | Daumas, Stéphanie | Fabre, Véronique

Edité par CCSD ; Elsevier Inc -

International audience. Exposure to stressors has profound effects on sleep that have been linked to serotonin (5-HT) neurons of the dorsal raphe nucleus (DR). However, the DR also comprises glutamatergic neurons expressing vesicular glutamate transporter type 3 (DRVGLUT3), leading us to examine their role. Cell-type-specific tracing revealed that DRVGLUT3 neurons project to brain areas regulating arousal and stress. We found that chemogenetic activation of DRVGLUT3 neurons mimics stress-induced sleep perturbations. Furthermore, deleting VGLUT3 in the DR attenuated stress-induced sleep perturbations, especially after social defeat stress. In the DR, VGLUT3 is found in subsets of 5-HT and non-5-HT neurons. We observed that both populations are activated by acute stress, including those projecting to the ventral tegmental area. However, deleting VGLUT3 in 5-HT neurons minimally affected sleep regulation. These findings suggest that VGLUT3 expression in the DR drives stress-induced sleep perturbations, possibly involving non-5-HT DRVGLUT3 neurons.

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