Syracuse

The MADS ‐box protein SlTAGL1 regulates a ripening‐associated SlDQD/SDH2 involved in flavonoid biosynthesis and resistance against Botrytis cinerea in post‐harvest tomato fruit

Archive ouverte

Wang, Ruochen | Liu, Kaidong | Tang, Bei | Su, Dan | He, Xiaoqing | Deng, Heng | Wu, Mengbo | Bouzayen, Mondher | Grierson, Don | Liu, Mingchun

Edité par CCSD ; Wiley -

International audience. 3‐Dehydroquinate dehydratase/shikimate dehydrogenase (DQD/SDH) is a key rate‐limiting enzyme that catalyzes the synthesis of the shikimate, which is an important metabolic intermediate in plants and animals. However, the function of SlDQD/SDH family genes in tomato ( Solanum lycopersicum ) fruit metabolites is still unknown. In the present study, we identified a ripening‐associated SlDQD/SDH member, SlDQD/SDH2 , that plays a key role in shikimate and flavonoid metabolism. Overexpression of this gene resulted in an increased content of shikimate and flavonoids, while knockout of this gene by CRISPR/Cas9 mediated gene editing led to a significantly lower content of shikimate and flavonoids by downregulation of flavonoid biosynthesis‐related genes. Moreover, we showed that SlDQD/SDH2 confers resistance against Botrytis cinerea attack in post‐harvest tomato fruit. Dual‐luciferase reporter and EMSA assays indicated that SlDQD/SDH2 is a direct target of the key ripening regulator SlTAGL1. In general, this study provided a new insight into the biosynthesis of flavonoid and B. cinerea resistance in fruit tomatoes.

• Description
• Sujet/Public
• Outil
• Outil d'anticipation
• Mémoire et thèse
• Gestion