The translational landscape of plant mitochondria and control by PPR proteins.

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Nguyen, Tan-Trung | Planchard, Noelya | Bertin, Pierre | Quadrado, Martine | Dargel-Graffin, Céline | Hatin, Isabelle | Namy, Olivier | Mireau, Hakim

Edité par CCSD -

International audience. Translation in plant mitochondria is a complex process that is still poorly understood at the molecular level. Growing evidence indicates though that mitochondrial translation differs from its bacterial counterpart in many key aspects. To better understand how mitochondrial translation is orchestrated and regulated in plants, we have used the ribosome profiling technology to generate genome-wide views of the mitochondrial translatome in different plant genotypes. This approach led us to reveal that most plant mitochondrial ribosome footprints measure 27 and 28 bases. Quantification of ribosome footprints along transcripts revealed that mRNAs have highly divergent ribosome densities, suggesting a tight control of translation initiation or elongation in plant mitochondria. To better understand the basis of this control, we identified and have been characterizing mitochondria-targeted pentatricopeptide repeat (PPR) proteins specifically involved in translation. I will present our most recent advances on the characterization of these PPR proteins.

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