Development of an oral nanovaccine for dogs against Echinococcus granulosus

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Leroux, Marine | Benavides, Uruguaysito | Hellel-Bourtal, Imène | Silvarrey, Cecilia | Milhau, Nadège | Marchal, Thierry | Bourgeois, Sandrine | Lawton, Philippe | Briançon, Stephanie | Petavy, Anne-Françoise | Lahmar, Samia | Esteves, Adriana | Almouazen, Eyad | Azzouz-Maache, Samira

Edité par CCSD ; Elsevier -

3/2. International audience. Dogs are the main source of animal and human cystic echinococcosis caused by the Cestode parasite Echinococcus granulosus. Dog vaccination seems to be a good strategy to control this parasitic disease. Here we present the development of a polymeric nanoparticle-based oral vaccine for dogs against Echinococcus granulosus delivered in enteric-coated capsules. To achieve our target, we encapsulated two recombinant antigens into biodegradable polymeric nanoparticles in the presence of Monophosphoryl lipid A as an adjuvant to ensure efficient delivery and activation of a protective mucosal immune response. The formulated delivery system showed a nanoparticle size less than 200 nm with more than 80 % antigen encapsulation efficiency and conserved integrity and immunogenicity. The nanoparticle surface was coated with chitosan to enhance adhesion to the gut mucosa and a subsequent antigen delivery. Chitosan-coated nanoparticles showed a higher cell internalization in murine macrophages and dendritic cells as well as a higher penetration into Caco-2 cells in vitro. Antigen-loaded nanoparticles were freeze-dried and enteric-coated capsules were filled with the obtained powder. The ob- tained results show a promising nanoparticles delivery system for oral vaccination.

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