Microsatellite markers developed through pyrosequencing allow clonal discrimination in the invasive alga Caulerpa taxifolia

0 avis

Microsatellite markers developed through pyrosequencing allow clonal discrimination in the invasive alga Caulerpa taxifolia

Archive ouverte

Arnaud-Haond, Sophie | Candeias, Rui | Serrao, Ester A. | Teixeira, Sara

Polymorphic microsatellites were developed for the invasive green algae Caulerpa taxifolia using next generation DNA sequencing. Results showed a limited rate of microsatellites for the amount of sequences, possibly explaining failure of previous attempts for microsatellite development through classical methods. Eight polymorphic loci were selected that exhibited polymorphism and a null or negligible rate of amplification failure. The number of alleles per locus ranged from two to seven. The reconstruction of Multi Locus Genotypes and the heterozygosity and departure from Hardy-Weinberg equilibrium confirmed the influence of clonal reproduction and showed the usefulness of this set of markers to successfully discriminate clonal lineages and analyze the clonal and genetic composition of algal beds. These markers will be used to further investigate the clonal composition and genetic structure in populations of Caulerpa taxifolia and to attempt retracing the origin of and pathways followed by invasive clonal lineages.

Langue: en

Consulter en ligne

Consulter le document

Suggestions

Du même auteur

High connectivity across the fragmented chemosynthetic ecosystems of the deep Atlantic Equatorial Belt: efficient dispersal mechanisms or questionable endemism?

Archive ouverte | Teixeira, Sara | CCSD

International audience. Chemosynthetic ecosystems are distributed worldwide in fragmented habitats harbouring seemingly highly specialized communities. Yet, shared taxa have been reported from highly distant chemosy...

Characterization of 15 polymorphic microsatellite loci in Rimicaris exoculata, and cross-amplification in other hydrothermal-vent shrimp

Archive ouverte | Teixeira, Sara | CCSD

Rimicaris exoculata is an alvinocarid shrimp endemic to the hydrothermal vents of the Mid-Atlantic Ridge. In order to study genetic variability and connectivity in this species, we developed fifteen polymorphic microsatellite mark...

Panmixia in a Fragmented and Unstable Environment: The Hydrothermal Shrimp Rimicaris exoculata Disperses Extensively along the Mid-Atlantic Ridge

Archive ouverte | Teixeira, Sara | CCSD

Dispersal plays a fundamental role in the evolution and persistence of species, and especially for species inhabiting extreme, ephemeral and highly fragmented habitats as hydrothermal vents. The Mid-Atlantic Ridge endemic shrimp s...

Chargement des enrichissements...