Afficher la couverture 'High‐Speed Cell Sorting of Infectious Trophic and Cystic Forms of Pneumocystis carinii | Martinez, Anna' en grand format
/5

0 avis

High‐Speed Cell Sorting of Infectious Trophic and Cystic Forms of Pneumocystis carinii

Archive ouverte

Martinez, Anna | Aliouat, El Moukhtar | Pottier, Muriel | Gantois, Nausicâa | Pinçon, Claire | Standaert-Vitse, Annie | Dei-Cas, Eduardo | Aliouat-Denis, Cécile‐marie

Edité par CCSD ; Wiley -

ABSTRACT. The separation of Pneumocystis carinii life‐cycle stages while preserving infectivity is a hitherto unresolved challenge. We describe an original, reproducible, and efficient method for separating trophic from cystic forms of P. carinii using a high‐speed cell sorter. The large amounts of highly purified (99.6±0.3%) infectious trophic and cystic forms can now be used to elucidate the poorly understood P. carinii life cycle.

Consulter en ligne

Suggestions

Du même auteur

SYTO-13, a Viability Marker as a New Tool to Monitor In Vitro Pharmacodynamic Parameters of Anti-Pneumocystis Drugs

Archive ouverte | Standaert-Vitse, Annie | CCSD

Relationship Between Pneumocystis carinii Burden and the Degree of Host Immunosuppression in an Airborne Transmission Experimental Model

Archive ouverte | Khalife, Sara | CCSD

Abstract To quantitatively assess the risk of contamination by Pneumocystis depending on the degree of immunosuppression (ID) of the exposed rat hosts, we developed an animal model, where rats went through different doses of dexam...

Growth and Airborne Transmission of Cell-Sorted Life Cycle Stages of Pneumocystis carinii

Archive ouverte | Martinez, Anna | CCSD

Chargement des enrichissements...