Adenine methylation is very scarce in the drosophila genome and not erased by the Ten Eleven Translocation dioxygenase

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Boulet, Manon | Gilbert, Guerric | Renaud, Yoan | Schmidt-Dengler, Martina | Auxerre-Plantié, Emilie | Bertrand, Romane | Nan, Xinsheng | Jurkowski, Tomasz | Helm, Mark | Vandel, Laurence | Waltzer, Lucas

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N6-methyladenine (6mA) DNA modification has recently been described in metazoans, including in drosophila, for which the erasure of this epigenetic mark has been ascribed to the Ten Eleven Translocation (TET) enzyme. Here, we re-evaluated 6mA presence and TET impact on drosophila genome. Using axenic or conventional breeding conditions, we found only traces of 6mA by LC-MS/MS and no significant increase in 6mA levels in the absence of TET. Further molecular and genetic analyses suggest that TET does not demethylate 6mA but acts essentially in an enzymatic-independent manner. Our results call for further caution concerning the role and regulation of 6mA DNA modification in metazoans.

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