Biofilm characterization of Clostridioides difficile strains isolated from Equidae

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Lemaire, Anaïs | Bridoux, Ludovic | Barbut, Frédéric | Petry, Sandrine | Poquet, Isabelle

Edité par CCSD -

International audience. Clostridioides difficile, a Gram-positive spore-forming strict anaerobe, is a One Health player. It is can infect both humans and animals and spread spores widely in the environment, finally allowing inter-species and zoonotic transmission. C. difficile is an opportunistic enteropathogen that can emerge after antibiotic treatment leading to microbiota dysbiosis. It is the primary agent of nosocomial diarrhoea worldwide and community-acquired infections are increasing, probably due to inter-species transmission. Biofilms could play a role in C. difficile infectious cycle. Our project is based on a collection of strains previously isolated from Equidae in our laboratories. The strains have been characterised at the molecular and phenotypic levels. The aim of our research is to characterise and compare strain ability to form biofilms. Biofilms are being analysed by direct observation, crystal violet staining and confocal scanning laser microscopy to measure their biomass and determine their morphology and structure. Preliminary results show few quantitative differences between strains according to biofilm biomass and some qualitative differences in biofilm morphology and structure. Our study contributes to a better understanding of biofilm formation in C. difficile according to strain diversity.

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