Afficher la couverture 'Towards the optimization of genetic polymorphism with EMS-induced mutagenesis in Phaeodactylum tricornutum | Rumin, Judith' en grand format
/5

0 avis

Towards the optimization of genetic polymorphism with EMS-induced mutagenesis in Phaeodactylum tricornutum

Archive ouverte

Rumin, Judith | Carrier, Gregory | Rouxel, Catherine | Charrier, Aurelie | Raimbault, Virginie | Cadoret, Jean-Paul | Bougaran, Gael | Saint-Jean, Bruno

Edité par CCSD ; Elsevier -

International audience. Microalgae remain an exciting target for biotechnology as they offer a largely unexploited reservoir of novel and valuable bioactive compounds. Strain improvement programs are an expanding research field aiming to multiply microalgal potential. This study evaluates the genetic diversity created in populations of the diatom Phaeodactylum tricornutum subjected to random mutagenesis. We explored the genetic diversity using genotyping-by-sequencing (GBS) to estimate and compare the impact of the most common chemical mutagen (ethyl methanesulfonate, EMS). Five microalga populations obtained following EMS treatment had survival rates between 1 and 98 %. High genetic diversity was obtained for only one of these P. tricornutum populations, with a survival rate close to 30 %.

Consulter en ligne

Suggestions

Du même auteur

Microalgae and Biotechnology. Microalgues et Biotechnologie

Archive ouverte | Cadoret, Jean-Paul | CCSD

High-affinity nitrate/nitrite transporter genes (Nrt2) in Tisochrysis lutea: identification and expression analyses reveal some interesting specificities of Haptophyta microalgae

Archive ouverte | Charrier, Aurelie Georgette | CCSD

International audience. Microalgae have a diversity of industrial applications such as feed, food ingredients, depuration processes and energy. However, microalgal production costs could be substantially improved by...

The use of fluorescent Nile red and BODIPY for lipid measurement in microalgae

Archive ouverte | Rumin, Judith | CCSD

International audience. Microalgae are currently emerging as one of the most promising alternative sources for the next generation of food, feed, cosmetics and renewable energy in the form of biofuel. Microalgae con...

Chargement des enrichissements...