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1H-NMR metabolomic study of Large White and Meishan pigs in late gestation: part 2 - sow endometrium
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Edité par CCSD -
International audience. The risk of piglet mortality is highest in the first few days after birth. Therefore,late gestation development is of high importance for piglet survival. The endometrium is thematernal tissue that is in direct contact with the placenta of each fetus and thus is the matrixfor feto-maternal interactions. To better understand the relationship between fetaldevelopment and piglet maturity, endometrial tissues from Large White (LW) sows, with highpiglet mortality, were compared to that from Meishan (MS) sows, a breed with less neonatalmortality. 224 endometrial samples were collected from 28 sows (14 LW and 14 MS) at 90 or110 days of gestation (dg, birth at 114 days), each sample being in direct contact with each ofthe 224 placentas. Hydrophilic metabolites were extracted and 1H-NMR spectra wereacquired. Raw spectra were processed using the ASICS R package to identify and quantify 46metabolites. A multivariate analysis revealed a main effect of gestational age (13.1% on 1staxis). Mixed models were used to identify 21 metabolites with differential concentrationsbetween the two following conditions, day of gestation and genotype, their interaction, andwith the sow as a random effect (one model fitted to each metabolite independently followedby a correction for multiple testing, FDR < 0.05). As expected, fructose was more abundant at90 dg than at 110 dg. Conversely, L-glutathione-reduced was more concentrated at 110 dgthan at 90 dg and more concentrated in MS than in LW sows. Citrate and L-glycine weremore concentrated in MS sows than in LW sows at both stages of gestation. A more detailedbiological interpretation of the metabolomic data is in progress. This research was part of theANR-20-CE20-0020-01 project COLOcATION.
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