0 avis
Evaluation of the fully automated LIAISON®XL chemiluminescence analyzer for QuantiFERON®-CMV testing in transplant recipients. Évaluation de l'analyseur par chimiluminescence LIAISON®XL entièrement automatisé pour le test QuantiFERON®-CMV chez les receveurs de greffe
Archive ouverte
International audience. Background: Monitoring CMV-specific cell-mediated immunity by the QuantiFERON®-CMV (QF-CMV) may beuseful in predicting the risk of CMV infection in transplant recipients (TR).Objectives: As the QuantiFERON-Tuberculosis (QFT®-Plus) became available on the fully automated LIAISON®XLchemiluminescence (CLIA) analyzer, we evaluated the performance of the QF-CMV on the LIAISON®XL analyzerusing the QuantiFERON®-TB Gold Plus reagent.Study design: Between 2018 and 2022, 81 samples from TR were collected at the Department of Virology ofLimoges Hospital, France. Whole blood was collected into each of the three QF-CMV collection tubes: a CMVantigentube (QF-Ag), a mitogen tube (QF-Mg) (positive control), and a nil tube (negative control). The QFCMVwas performed on the LIAISON®XL analyzer, and results were compared with those obtained by conventionalmicroplate ELISA.Results: Intra- and inter-assay coefficients of variation were inferior to 20%. No inter-sample contamination wasfound (p=0.366). The level of concordance between CLIA and the commonly used ELISA method was 88.9%.Positive and negative percent agreements were 92.3% and 85.7%, respectively, with a very good agreementbetween assays (κ=0.818). Most discordances were due to indeterminate- or negative-ELISA/positive-CLIA results(most of ELISA results were borderline). Linear regression analyses demonstrated a strong correlation betweenboth assays (QF-Ag Pearson’s r=0.978, QF-Mg Pearson’s r=0.963). No significant difference was observedin median QF-CMV values between both assays (QF-Ag p=0.776; QF-Mg p=0.853; Mann-Whitney U test). TheBland-Altman plots showed a minor difference in IFN-γ release (QF-Ag -0.069 IU/ml, 95% limits of agreement(LoA): -1.589; 1.451; QF-Mg 0.190 IU/ml, 95% LoA: -2.070; 2.450).Conclusion: Automated QF-CMV with CLIA is comparable to QF-CMV performed by ELISA with a presumablyhigher sensitivity for IFN-γ detection that may result in the conversion of samples close to the ELISA cut-off intopositive results. Moreover, the use of a random-access analyzer allows to optimize the follow-up of TR.
Consulter en ligne
Suggestions
Du même auteur
