Syracuse

Evaluation of the Unyvero i60 ITI® multiplex PCR for infected chronic leg ulcers diagnosis

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Reissier, Sophie | Lazareth, Isabelle | Adjiman, Louise | Couzigou, Carine | Vidal, Barbara | Mizrahi, Assaf | Nguyen Van, Jean Claude | Pilmis, Benoît | Le Monnier, Alban | Lourtet-Hascoët, Julie

Edité par CCSD -

International audience. Objectives: Unyvero i60 ITI multiplex PCR (mPCR) may identify a large panel of bacteria and antibiotic resistance genes. In this study, we compared results obtained by mPCR to standard bacteriology in chronic leg ulcer (CLU) infections.Methods: A prospective study, part of the interventional-blinded randomized study "ulcerinfecte" (NCT02889926), was conducted at Saint Joseph Hospital in Paris. Fifty patients with a suspicion of infected CLU were included between February 2017 and September 2018. Conventional bacteriology and mPCR were performed simultaneously on deep skin biopsies.Results: Staphylococcus aureus and Pseudomonas aeruginosa were the most detected pathogens. Regarding the global sensitivity, mPCR is not overcome to the standard culture. Anaerobes and slow growing bacteria were detected with a higher sensitivity rate by mPCR than standard culture.Conclusion: Unyvero i60 ITI multiplex PCR detected rapidly pathogenic bacteria in infected CLU especially anaerobes and slow growing bacteria and was particularly effective for patients previously treated with antibiotics.

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